Joint Macromolecular Crystallography Workshop: Using the Uni-Puck and Web-Ice at ALS and SSRL
A joint workshop will be held on Oct 10 at the ALS and on Oct 11 at SSRL focusing on collaborative developments in automated sample screening and rapid crystal assessment. The aim of the workshop is to familiarize users with the Uni-Puck and the use of the Web-Ice data analysis software.
Although the majority of automated sample mounting systems are compatible with Hampton-style magnetic cryo-pins, many types of containers are used for sample pin transportation and storage. To simplify sample preparation for the synchrotron user community, a collaboration was formed between the ALS, APS SBC-CAT, and SSRL to develop the Uni-Puck, a common sample pin storage and shipping container compatible with the robotic sample mounting systems used at all of these facilities. Workshop participants will gain hands-on experience using the newly designed Uni-Puck with both the ALS and SSRL robotic sample mounting systems.
Web-Ice is a web browser-based facility for diffraction image viewing, image analysis, autoindexing and strategy calculation developed at SSRL and in collaboration with LBNL. Workshop participants will learn how to use Web-Ice to display screening results, index diffraction patterns and set up strategies for data collection.
At the ALS a series of talks on the morning of the first day will provide background material, and technical details of the Uni-Puck and Web-Ice. The afternoon of the same day users will be able to gain experience using the puck in the Berkeley Automounter at the ALS sector 5.0 beamlines. The following day, in the morning at SSRL, users will use the Uni-Puck in the Stanford Automounter. In the afternoon the Web-Ice software will be demonstrated.
Organizers:
Paul Adams, Aina Cohen, Nicholas Sauter, Michael Soltis, and Christine Trame
Location: ALS and SSRL